The two mains projects are the development of innovative skin models reproducing cutaneous microenvironment and/or specific epithelia and the study of skin aging mechanism to build new strategies for its prevention/treatment.
Development of innovative skin models
Our studies on physioxia have highlighted the importance of reconstituting physiological skin microenvironment for cutaneous biology studies. The characterization of pathways affected by oxygen level is investigated at the molecular level by studying transcriptome and miRnome with the aim to define optimized 3D models to replace animal use. We intend to use skin models grown in physioxia to determine more pertinent molecular targets for dermatology and cosmetology purposes in collaboration with cosmetic industries.
We develop vulvar tissue 3D models including Langerhans cells and endothelial cells. Transcriptome including miRNome mapping of vulvar tissue established from biopsies is used to validate them. The impact of the microenvironment (mucin production, commensal bacterial microflora), mechanical stress, inflammation and aging physiology will be assessed on these models. Better understanding of the cellular and molecular mechanisms involved in the pathology will contributed to the development of cosmetics and pharmaceutics accurate for this unique tissue.
Skin aging biology and regeneration
Studies are conducted on the role of microRNA in skin aging biology by evaluating their impact on the regulation of pigmentation, extracellular matrix production and anti-oxidant defenses. We also decipher whether such exosomes containing microRNA could play a role in cell-cell communications regulating skin homeostasis. To prevent or treat skin aging/injury, novel strategies are set up to exploit microRNAs regulation, glycosylated compounds, bioactive molecules. Most of these studies involved private partners. New project aims to evaluate the potential effects of non-thermal cold plasma to treat skin aging damages.
Significant publications :
- Nadim M., Hassanaly S., Dubannet L. and Grillon C.
Physioxia and microRNAs as key factors in the skin microenvironmentarticle. International Federation of Societies of Cosmetic Chemists (2015) 18 (1) 35-43
- Nadim, M. Auriol, D. Lamerant-Faye, L. N. Lefevre, F. Dubanet, L. Redziniak, G. Kieda and C. Grillon, C.
Improvement of polyphenol properties upon glucosylation in a UV-induced skin cell ageing modelarticle. International journal of cosmetic science (2014) 36 (6) 579-587
- Grillon C., Matejuk A., Nadim M., Lamerant-Fayel N. and Kieda C.
News on microenvironmental physioxia to revisit skin cell targeting approachesarticle. Exp Dermatology (2012) 21 (10) 723-728
- Carreau, A., El Hafny-Rahbi, B., Matejuk, A., Grillon, C. and Kieda, C.
Why is the partial oxygen pressure of human tissues a crucial parameter ? J. Cell. Molecular Medecine (2011) 15 (6) 1239-1253
- Carreau A., Kieda C. and Grillon C.
Nitric oxide modulates endothelial cell adhesion molecules involved in angiogenesis and leukocyte recruitment. Exptl. Cell (2011) 317 (1) 29-41