Accueil > Publications > Recherche par années > Années 1990 > 1991

LeDoan, T ; Perrouault, L ; Asseline, U ; Thuong, NT ; Rivalle, C ; Bisagni, E ; Helene, C

Recognition and photoinduced cleavage and cross-linking of nucleic-acids by oligonucleotides covalently linked to ellipticine

Antisense Research and Development 1 (1) 43-54

par Administrateur - publié le

Abstract :

Oligopyrimidines covalently linked to ellipticine derivatives form duplex and triplex structures with target single-stranded oligopurine sequences. They also bind to duplex DNA at homopurine-homopyrimidine sequences where they form local triple helices. Irradiation at wavelengths longer than 300 nm of the complex formed by an oligonucleotide-ellipticine conjugate with its target sequence induced (i) cleavage of the target at bases located in close proximity to the dye and (ii) cross-linking of the target sequence to the derivatized oligonucleotide. Both cross-linking and cleavage reactions decreased when temperature increased with a half-transition corresponding to the dissociation of the oligonucleotide-ellipticine conjugate from its target nucleic acid, demonstrating that the observed photochemical effects are dependent on hybrid formation. When the target was a double-stranded DNA, photochemical reactions were observed on both strands of the duplex. Photo-induced cross-linking was more efficient than cleavage when the target was single-stranded ; the reverse was observed when the target was duplex DNA.