Accueil > Publications > Recherche par années > Années 1990 > 1991

Slim, G ; Pritchard, C ; Biala, E ; Asseline, U ; Gait, MJ

Synthesis of site-specifically modified oligoribonucleotides for studies of the recognition of TAR RNA by HIV-1 tat protein and studies of hammerhead ribozymes.

Nucleic Acids Symposium Series (24) 55-58

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Abstract :

Synthetic oligoribonucleotides having single uracil residues replaced by dU, dT, 2’-O-methylU or 5-bromodU have been prepared and used in the study of the interaction of HIV-1 tat protein with an RNA stem-loop. The preparation of phosphoramidites of 5-bromouridine and purine riboside suitable for use in solid-phase oligoribonucleotide synthesis is also described. The effect of adenine replacement by purine in a hammerhead ribozyme has also been determined.