Slim, G ; Pritchard, C ; Biala, E ; Asseline, U ; Gait, MJ
Nucleic Acids Symposium Series (24) 55-58
publié le , mis à jour le
Synthetic oligoribonucleotides having single uracil residues replaced by dU, dT, 2’-O-methylU or 5-bromodU have been prepared and used in the study of the interaction of HIV-1 tat protein with an RNA stem-loop. The preparation of phosphoramidites of 5-bromouridine and purine riboside suitable for use in solid-phase oligoribonucleotide synthesis is also described. The effect of adenine replacement by purine in a hammerhead ribozyme has also been determined.