Accueil > Publications > Recherche par années > Années 1990 > 1991

Jamin, M ; Adam, M ; Damblon, C ; Christiaens, L ; Frère, JM

Accumulation of acyl-enzyme in DD-peptidase-catalyzed reactions with analogs of peptide-substrates

Biochemical Journal 280 499-506

par Administrateur - publié le

Abstract :

Thioester substrates can be used to study the hydrolysis and transfer reactions catalysed by ß-lactamases and DD-peptidases. With the latter enzymes, accumulation of the acyl-enzyme can be detected directly. The efficiency of various amines as acceptor substrates was in excellent agreement with previous results obtained with peptide substrates of the DD-peptidases. The results indicated the presence of a specific binding site for the acceptor substrates, Although most of the results agreed well with a simple partition model, more elaborate hypotheses will be needed to account for all the data presented.