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Accueil > Publications > Recherche par années > Années 1990 > 1991

Pimpaneau, V ; Midoux, P ; Monsigny, M ; Roche, AC

Characterization and isolation of an intracellular d-mannose-specific receptor from human promyelocytic-hl-60 cells

Carbohydrate Research 213 95-108

par Administrateur - publié le

Abstract :

Most mammalian macrophages express D-mannose-specific receptor (membrane lectin, M(r) 175000) allowing endocytosis of their ligands, but cells of the monocytic lineage (HL60, U937, monocyte) lack this receptor. However, after permeabilization, promyelocytic, promonocytic cells and monocytes bound fluoresceinylated D-mannose-terminated neoglycoproteins as evidenced by flow cytometry. Under these conditions, confocal analysis confirmed the intracellular membrane localization of the labeling and the absence of nuclear binding. An intracellular D-mannose-specific receptor was isolated from the human promyelocytic cell line HL60, by affinity chromatography on 4-isothiocyanatophenyl a-D-Mannopyranoside-substituted Affi-gel as a 60 000-M(r) membrane protein requiring divalent cations for the ligand binding. Under the same conditions, mouse macrophages were shown to express a 175 000-M(r) D-Mannose-specific receptor but not the 60 000-M(r) receptor.