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Accueil > Publications > Recherche par années > Années 1990 > 1992

Lancelot, G ; Gervais, A ; Maurizot, JC

2-dimensional H-1, N-15 NMR investigation of uniformly N-15-labeled lac repressor headpiece

Journal of Biomolecular Structure & Dynamics 9 (5) 921-933

par Administrateur - publié le

Abstract :

N-15 uniformly labeled lac repressor and lac repressor headpiece were prepared. N-15 NMR spectra of lac repressor were shown resolution inadequate for detailed study while the data showed that the N-15 labeled N-terminal part of the protein is quite suitable for this type of study allowing future investigation of the specific interaction of the lac repressor headpiece with the lac operator. We report here the total assignment of proton H-1 and nitrogen (NH)-N-15 backbone resonances of this headpiece in the free state. Assignments of the N-15 resonances of the protein were obtained in a sequential manner using heteronuclear multiple quantum coherence (HMQC), relayed HMQC nuclear Overhauser and relayed HMQC-HOHAHA spectroscopy.