Accueil > Publications > Recherche par années > Années 1990 > 1993

Durand, M ; Maurizot, JC ; Asseline, U ; Thuong, NT ; Helene, C

Oligo-a-thymidylates covalently-linked to intercalating agents - circular-dichroism studies of their interaction with complementary sequences

Bioconjugate Chemistry 4 (3) 206-211

par Administrateur - publié le

Abstract :

The interaction between oligo-a-thymidylates covalently linked to an intercalating agent (an acridine derivative) and their complementary sequences containing ß-nucleosides (poly(rA), poly(dA), r(Ap)7rA, p(dA)8) has been studied using circular dichroism spectroscopy. Binding to poly(rA) and to poly(dA) of the modified oligonucleotides led to large changes in the induced circular dichroism signal of the acridine ring. These changes depend on whether the dye is linked to the 3’- or to the 5’-end of the oligonucleotide. Interaction with poly(rA) as well as interaction with an octadeoxyriboadenylate led to the formation of a 1A:1T complex.