Accueil > Publications > Recherche par années > Années 1990 > 1993

Naim, M ; Charpentier, S ; Nicolas, P ; Baron, D

Quantitative two-dimensional NMR study of dermenkephalin, a highly potent and selective d-opioid peptide

Biopolymers 33 (12) 1889-1900

par Administrateur - publié le

Abstract :

Dermenkephalin, H-Tyr-(D)Met-Phe-His-Leu-Met-Asp-NH2, a highly potent and selective d-opioid peptide isolated from frog skin, was studied in DMSO-d(6) solution by two-dimensional nmr spectroscopy, including the determination of NH temperature coefficients, the evaluation of (3)J coupling constants from phase-sensitive correlated spectroscopy (COSY) and the volumes of nuclear Overhauser effect (NOE) correlations. The two-dimensional NOE spectroscopy (NOESY) spectrum of dermenkephalin revealed sequential, medium-, and long-range effects. To put this information on a quantitative basis, special attention was devoted to J cross-peak suppression, quantification of the NOE volumes and analysis of the overlaps, normalization of the NOEs against diagonal peaks and H beta beta, geminal interactions.