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Wdzieczak-Bakala, J ; Grillon, C ; Robinson, S ; Riches, A ; Carde, P ; Lenfant, M

Catabolism of the tetrapeptide N-Ac-Ser-Asp-Lys-Pro (AcSDKP), an inhibitor of hematopoietic stem-cell (CFU-S) proliferation, following in vitro incubation with hematopoietic tissues from normal and leukemic mice

Bulletin Du Cancer 80 (5) 391-396

par Administrateur - publié le , mis à jour le

Abstract :

The comparative degradation of N-Ac-Ser-Asp-Lys-Pro (AcSDKP), a negative regulator controlling the proliferation of the hematopoietic pluripotent stem cell, was investigated following incubation with plasma, bone marrow and spleen cells from normal mice and mice bearing a transplantable myeloid leukemia. Using the tetrapeptide, specifically radiolabelled in the lysyl residue, degradation of [H-3]AcSDKP was followed by measurement of [H-3]Lys formation resulting from its catabolism. It was shown that already after 1 h the degradation of AcSDKP in plasma from leukemic mice was higher compared to that following incubation in plasma from normal mice, whereas incubation with bone marrow cells exhibits a small difference only after 4 hours incubation. However, no increase of AcSDKP catabolic activity was observed following incubation with spleen cells from leukemic animals when compared with incubation of normal spleen cells.