Accueil > Publications > Recherche par années > Années 1990 > 1993

Grillon, C ; Lenfant, M ; Wdzieczak-Bakala, J

Optimization of cell-culture conditions for the evaluation of the biological-activities of the tetrapeptide N-Acetyl-Ser-Asp-Lys-Pro, a natural hemoregulatory factor

Growth Factors 9 (2) 133-138

par Administrateur - publié le , mis à jour le

Abstract :

The present study attempts to define the difficulties in evaluating the properties of the hemoregulatory peptide AcSDKP using in vitro assays. In fact, in the presence of sera, which are generally added to basic culture media, AcSDKP is catabolized by proteases present in the serum. The kinetics of AcSDKP degradation depends on the nature and on the concentration of the added serum. In in vitro conditions, the half life of this peptide can be increased by the addition of 1 muM captopril, a metalloprotease inhibitor. Thus, these points need to be considered in designing experiments to study the effects of AcSDKP.