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Accueil > Publications > Recherche par années > Années 1990 > 1993

Midoux, P ; Mendes, C ; Legrand, A ; Raimond, J ; Mayer, R ; Monsigny, M ; Roche, AC

Specific gene-transfer mediated by lactosylated poly-l-lysine into hepatoma-cells

Nucleic Acids Research 21 (4) 871-878

par Administrateur - publié le

Abstract :

Plasmid DNA/glycosylated polylysine complexes were used to transfer in vitro a luciferase reporter gene into human hepatoma cells by a receptor-mediated endocytosis process. HepG2 cells which express a galactose specific membrane lectin were efficiently and selectively transfected with pSV2Luc/lactosylated polylysine complexes in a sugar dependent manner : i) HepG2 cells which do not express membrane lectin specific for mannose were quite poorly transfected with pSV2Luc/mannosylated polylysine complexes, ii) HeLa cells which do not express membrane lectin specific for galactose were not transfected with pSV2Luc/lactosylated polylysine complexes.