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Accueil > Publications > Recherche par années > Années 1990 > 1993

Elmoudni, B ; Philippe, M ; Monsigny, M ; Schrevel, J

N-acetylglucosamine-binding proteins on plasmodium-falciparum merozoite surface

Glycobiology 3 (4) 305-312

par Administrateur - publié le

Abstract :

Plasmodium falciparum merozoite surface is specifically labelled with a neoglycoprotein bearing N-acetylglucosamine (GlcNAc) residues in a sugar-dependent manner, as shown by affinity cytochemistry in fluorescence and electron microscopy. To ascertain the nature of the sugar receptor, merozoite proteins were blotted and tested by a two-step method using biotinylated GlcNAc-bovine serum albumin (BSA) and streptavidin-peroxidase conjugate. Three parasite proteins were specifically revealed and designated as Pf 120, Pf 83 and Pf 45 GlcNAc-binding proteins. These proteins bind to a gel substituted with GlcNAc and are specifically eluted with 300 mM GlcNAc.