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Accueil > Publications > Recherche par années > Années 1990 > 1994

Bourgerie, S ; Berger, S ; Strecker, G ; Julien, R ; Karamanos, Y

A fluorescence high-performance liquid-chromatography assay for enzymes acting on the di-n-acetylchitobiosyl part of asparagine-linked glycans

Journal of Biochemical and Biophysical Methods 28 (4) 283-293

par Administrateur - publié le , mis à jour le

Abstract :

The glycoasparagine, Man(7)GlcNAc(2)Asn (’Man(7)’) was labelled with resorufin and used as a specific substrate for the detection and quantification of endo-beta-N-acetyl glucosaminidases (Endos) acting on the di-N-acetylchitobiosyl part of asparagine-linked glycans. Peptide-N-4-(N-acetyl-beta-glucosaminyl) asparagine amidases (PNGases) cannot transform this substrate but they can be detected by the procedure described earlier using the resorufin-labelled N-glycopeptide [Glycoconjugate J., 9 (1992) 162-167]. These two substrates can be used in a simple, reproducible and very sensitive fluorescence HPLC assay in order to monitor Endo and PNGase activities during isolation and purification processes, or studies of the evolution of such activities during cultivation of the producing cells.