Accueil > Publications > Recherche par années > Années 1990 > 1994

Bourgerie, S ; Karamanos, Y ; Grard, T ; Julien, R

Purification and characterization of an endo-n-acetyl-beta-d-glucosaminidase from the culture-medium of stigmatella-aurantiaca dw4

Journal of Bacteriology 176 (20) 6170-6174

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Abstract :

A novel endo-N-acetyl-beta-D-glucosaminidase (ENGase), acting on the di-N-acetylchitobiosyl part of N-linked glycans, was characterized in the culture medium of Stigmatella aurantiaca DW4. Purified to homogeneity by ammonium sulfate precipitation, gel filtration, and chromatofocusing, this ENGase presents, upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, a molecular mass near 27 kDa. Optimal pH and pI were 4.0 and 6.8, respectively. The enzyme, named ENGase St, exhibits high activity on oligomannoside-type glycoasparagines and glycoproteins and could also hydrolyze hybrid- and complex-type glycoasparagines but does not acts as a murein hydrolase.