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Accueil > Publications > Recherche par années > Années 1990 > 1994

Lakaye, B ; Damblon, C ; Jamin, M ; Galleni, M ; Lepage, S ; Joris, B ; Marchandbrynaert, J ; Frydrych, C ; Frère, JM

Synthesis, purification and kinetic-properties of fluorescein-labeled penicillins

Biochemical Journal 300 141-145

par Administrateur - publié le

Abstract :

The synthesis and properties of six fluorescein-labelled penicillins are reported. The two isomers of fluoresceyl-glycyl-6-amino-penicillanic acid are probably the best compounds to use for detection of all the penicillin-binding proteins (PBPs) present in a bacterial membrane preparation. However, the derivatives of ampicillin were much more efficient against Enterobacter aerogenes PBP3. The two isomers obtained when a commercial mixture of the two isomers of carboxyfluorescein was used most often exhibited similar properties, but the Streptomyces R61 extracellular DD-peptidase was only efficiently acylated by the 5’-carboxyfluorescein derivative of glycyl-6-aminopenicillanic acid.