Accueil > Publications > Recherche par années > Années 1990 > 1994

Lancelot, G ; Maurizot, JC ; Chassignol, M ; Thuong, NT ; Chanteloup, L ; Beau, JM

DNA-protein interaction in solution - NMR-studies of the C-13-site-specifically labeled lac operator lac repressor complex

Magnetic Resonance in Chemistry 32 (10) 605-607

par Administrateur - publié le

Abstract :

Carhon-13 and proton resonance assignments for a DNA fragment complexed with its regulatory protein were obtain with the use of a selectively C-13-labelled oligonucleotide. Data obtained on the Lac operator-lac repressor complex from the HMQC-NOESY maps of the free and complexed Lac operator demonstrate that only minor conformational modifications are induced on complexation.