Accueil > Publications > Recherche par années > Années 1990 > 1994

Rouleux, F ; Monsigny, M ; Legrand, A

A negative regulatory element of the macrophage-specific human mannose receptor gene represses its expression in nonmyeloid cells

Experimental Cell Research 214 (1) 113-119

par Administrateur - publié le

Abstract :

We have cloned the putative promoter of the human mannose receptor gene using the ligation-mediated polymerase chain reaction. This modified polymerase chain reaction method depends upon the ligation of restricted genomic DNA fragments to a sequence of DNA containing a generic primer site. Approximately 400 bp of genomic DNA sequence immediately upstream from the 5’ end of the lectin gene was amplified with this strategy. Primer-extended reverse transcription identified several 5’ ends of the mannose receptor mRNA corresponding to differential use of initiation transcription sites.