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Accueil > Publications > Recherche par années > Années 1990 > 1994

Bizouarne, N ; Denis, V ; Legrand, A ; Monsigny, M ; Kieda, C

A SV40 immortalized murine endothelial-cell line from peripheral lymph-node high endothelium expresses a new a-L-fucose binding-protein

Biology of The Cell 79 (3) 209-218

par Administrateur - publié le

Abstract :

Endothelial cells from mouse peripheral lymph nodes were immortalized by cationic liposome-mediated transfection using a plasmid construct containing both the gene coding for the large T antigen of simian virus 40 and a geneticin resistance gene suitable for selection. A cell line (HECa10) was isolated on the basis of its capacity to specifically bind fucoside carrying glycoconjugates ; these cells present the main characteristics of endothelial cells : production of angiotensin converting enzyme and of factor VIII-related antigen.