Accueil > Publications > Recherche par années > Années 1990 > 1994

Malinge, JM ; Perez, C ; Leng, M

Base sequence-independent distorsions induced by interstrand cross-links in cis-diaminedichloroplatinum (II)-modified DNA

Nucleic Acids Research 22 (19) 3834-3839

par Administrateur - publié le

Abstract :

Physico-chemical and immunological studies have been done in order to further characterize the distorsions induced in DNA by the interstrand crosslinks formed between the antitumor diamminedichloroplatinum (II) (cis-DDP) guanines on the opposite strands of DNA at the d(GC/GC) sites. Bending (45 degrees) and unwinding(79 4 degrees) were determined from the electrophoretic mobility of multimers of 21- 24-base pairs double-stranded oligonucleotides containing an interstrand cross-link in the central sequence d(TGCT/AGCA).