Accueil > Publications > Recherche par années > Années 1990 > 1994

Cros, P ; Kurfurst, R ; Allibert, P ; Battail, N ; Piga, N ; Roig, V ; Thuong, NT ; Mandrand, B ; Helene, C

Monoclonal-antibodies targeted to ?-oligonucleotides - characterization and application in nucleic-acid detection

Nucleic Acids Research 22 (15) 2951-2957

par Administrateur - publié le

Abstract :

The aim of the present study was to test the antigenicity of a-deoxyribonucleotides in order to develop a new tool for the detection of nucleic acid sequences for use in diagnostic applications. We describe four monoclonal antibodies (Mabs) which recognize a-deoxyribonucleotides. Two were raised against a poly(a-dT) sequence and specifically recognized the a-dT nucleotide. Two were raised against a sequence containing all four common nucleotides as a-nucleotides and, surprisingly, only recognized the a-dG nucleotide. For all four Mabs, no cross reactivity was observed with ß-oligonucleotides.