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Accueil > Publications > Recherche par années > Années 1990 > 1995

Boudvillain, M ; Dalbies, R ; Aussourd, C ; Leng, M

Intrastrand cross-links are not formed in the reaction between transplatin and native dna - relation with the clinical inefficiency of transplatin

Nucleic Acids Research 23 (13) 2381-2388

par Administrateur - publié le

Abstract :

The reaction between trans-diamminedichloroplatinum(II) and single-stranded oligonucleotides containing the sequence d(GXG) (X being an adenine, cytosine or thymine residue) yields trans(Pt(NH3)(2) [d(GXG)-GN7,GN7]) intrastrand cross-links, These cross-links do not prevent the pairing of the platinated oligonucleotides with their complementary strands but they decrease the thermal stability of the duplexes, The thermal stability is not much affected by the chemical nature of the X residue and its complementary base, By gel electrophoresis, it is shown that the trans- Pt(NH3)(2)[d(GTG)-GN7,GN7] cross-link bends the DNA double helix (26 degrees) and unwinds it (45 degrees), The pairing of the platinated oligonucleotides with their complementary strands promotes the rearrangement of the 1,3-intrastrand cross-links into interstrand cross-links.