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Accueil > Publications > Recherche par années > Années 1990 > 1995

Gaudin, F ; Paquet, F ; Chanteloup, L ; Beau, JM ; Thuong, NT ; Lancelot, G

Selectively C-13-enriched DNA - dynamics of the C1’-H1’ vector in D(CGCAAATTTGCG)(2)

Journal of Biomolecular NMR 5 (1) 49-58

par Administrateur - publié le

Abstract :

In order to examine the internal dynamic processes of the dodecamer d(CGCAAATTTGCG)(2), the C-13- enriched oligonucleotide has been synthesized. The three central thymines were selectively C-13-labeled at the C1’ position and their spin-lattice relaxation parameters R(C-Z), R(C-X,C-Y), R(H-Z—>C-Z), R(HZCZ), R(2H(Z)C(X,Y)) and R(H-Z(C)) were measured. Density functions were computed for two models of internal motions. Comparisons of the experimental data were made with the spin-lattice relaxation rates rather than with the density functions, whose values were altered by accumulation of the uncertainties of each relaxation rate measurement. The spin-lattice relaxation rates were computed with respect to the motions of the sugar around the C1’-N1 bond.