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Erbacher, P ; Roche, AC ; Monsigny, M ; Midoux, P

Glycosylated polylysine DNA complexes - gene-transfer efficiency in relation with the size and the sugar substitution level of glycosylated polylysines and with the plasmid size

Bioconjugate Chemistry 6 (4) 401-410

par Administrateur - publié le

Abstract :

A DNA delivery system based on the use of polylysine substituted with small recognition signals, such as carbohydrate moieties specifically recognized by membrane lectins present in a given cell line, has been developed [Midoux et al. (1993) Nucleic Acids Res. 21, 871-878]. Human hepatoma (HepG2) cells which express a galactose-specific membrane lectin are efficiently transfected in the presence of chloroquine with pSV2Luc plasmid complexed with a lactosylated polylysine.