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Midoux, P ; Mayer, R ; Monsigny, M

Membrane permeabilization by a-helical peptides - a flow-cytometry study

Biochimica et Biophysica Acta-Biomembranes 1239 (2) 249-256

par Administrateur - publié le

Abstract :

The permeabilization by a-helical peptides of nucleated mammalian cells can be monitored by flow cytometry. Ethidium bromide, a non fluorescent and poorly membrane permeant molecule, becomes strongly fluorescent only upon binding to DNA. On this basis, the permeabilization of the plasma membrane of HL60 promyelocytic cells induced by a-helical peptides such as melittin, succinylated melittin and anionic peptides derived from the N-terminus of HA2 subunit of the influenza virus hemagglutinin, was measured. Melittin (GIGAVLKVLTTGLPALISWIKRKRQQ-NH2) caused a rapid (