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Girard, PM ; Bonnetmathoniere, B ; Muriaux, D ; Paoletti, J

A short autocomplementary sequence in the 5’-leader region is responsible for dimerization of momulv genomic RNA

Biochemistry 34 (30) 9785-9794

par Administrateur - publié le

Abstract :

Previous work has shown that a region of Moloney murine leukemia virus (MoMuLV) RNA located between nucleotides 280 and 330 in the PSI region (nt 215-565) is implicated in the dimerization process. We show with a deletion from nucleotides 290-299 in PSI RNA transcripts and through an antisense oligonucleotide complementary to nucleotides 275-291 that the 283-298 region is involved in RNA dimer formation in vitro. In an attempt to further characterize the mechanism of dimer formation, a series of short RNA transcripts was synthesized which overlapps the PSI region of MoMuLV RNA.