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Perche Federico


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tél : 02.38.25.56.02 - fax : 02.38.25.78.07

Publications

2012   Références trouvées : 1

Perche, F. Lambert, O. Berchel, M. Jaffrès, P.A. Pichon, C. Midoux, P.  (2012)

Gene transfer by histidylated lipopolyplexes : A dehydration method allowing preservation of their physicochemical parameters and transfection efficiency

International Journal of Pharmaceutics 423 (1) 144-150
Lipid-Polycation-DNA complexes (LPD) is a promising non-viral system for nucleic acids delivery. Usually, LPD are prepared just before their use. In the present work, we have examined whether dehydration of a new type of LPD (named LPD100) might be a storage option. LPD100 comprises PEGylated histidylated polylysine/pDNA polyplexes and a liposomal formulation made with lipophosphoramidates containing N-methylimidazolium and histamine polar heads. LPD100 were dehydrated by evaporation, and the physicochemical parameters and transfection efficiency (TE) of reconstituted LPD100 were compared to that of fresh LPD100. LPD100 previously dehydrated in the presence of 20% saccharose, displayed comparable size and surface charge as freshly prepared LPD100 but gave a better TE. CryoTEM experiments showed that the reconstituted LPD100 exhibited a shape similar to fresh ones. Moreover, when LPD100 were prepared with dehydrated pDNA/polymer complexes and fresh liposomes, TE was as efficient as with fresh LPD100 while a small increase of their size were observed. These results demonstrate that evaporation of LPD100 in the presence of saccharose is a powerful method to store them for a long period of time.

Lipid-Polycation-DNA complexes (LPD) is a promising non-viral system for nucleic acids delivery. Usually, LPD are prepared just before their use. In the present work, we have examined whether dehydration of a new type of LPD (named LPD100) might be a storage option. LPD100 comprises PEGylated histidylated polylysine/pDNA polyplexes and a liposomal formulation made with lipophosphoramidates containing N-methylimidazolium and histamine polar heads. LPD100 were dehydrated by evaporation, and the physicochemical parameters and transfection efficiency (TE) of reconstituted LPD100 were compared to that of fresh LPD100. LPD100 previously dehydrated in the presence of 20% saccharose, displayed comparable size and surface charge as freshly prepared LPD100 but gave a better TE. CryoTEM experiments showed that the reconstituted LPD100 exhibited a shape similar to fresh ones. Moreover, when LPD100 were prepared with dehydrated pDNA/polymer complexes and fresh liposomes, TE was as efficient as with fresh LPD100 while a small increase of their size were observed. These results demonstrate that evaporation of LPD100 in the presence of saccharose is a powerful method to store them for a long period of time.


2011   Références trouvées : 2

Perche, F., Benvegnu, T., Berchel, M., Lebègue, L., Pichon, C., Jaffrès, P.-A. & Midoux, P.,  (2011)

Enhancement of dendritic cells transfection in vivo and of vaccination against B16F10 melanoma with mannosylated histidylated lipopolyplexes loaded with tumor antigen messenger RNA

Nanomedicine : Nanotechnology, Biology and Medicine 7 (4) 445-453

Perche, F., Gosset, D., Mével, M., Miramon, M.-L., Yaouanc, J.J., Pichon, C., Benvegnu, T., Jaffrès, P.A. & Midoux, P.  (2011)

Selective delivery in dendritic cells with Mannosylated and Histidylated Lipopolyplexes.

J. Drug Target. 19 (5) 315-325


Mots-clés

Doctorant , Thérapies innovantes et nanomédecine