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Accueil > Publications > Recherche par années > Années 1990 > 1996

Verge, V ; Legrand, A ; Hevor, T

Isolation of carbohydrate metabolic clones from cultured astrocytes

Glia 18 (3) 244-254

par Administrateur - publié le

Abstract :

Astrocytes are the principal sites of glycogen synthesis in the nervous tissue. Growing evidence shows that there are many types of astrocytes. The aim of the present investigation was to isolate different types of astrocytes that display different carbohydrate anabolism. Astrocytes from newborn rat brain were directly cloned from primary cultures without a previous transformation. Many clones were obtained, and they were termed CP clones. Another series of clones, termed SV clones, were obtained after the transfection of the primary cultures by the SV40 T antigen.