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Accueil > Publications > Recherche par années > Années 1990 > 1996

Hebert, E ; Roche, AC ; Nachtigal, M ; Monsigny, M

Transformation but not ras-transfection increases the expression of galectin-3 in human HOS cells

Comptes Rendus de L Academie Des Sciences Serie Iii-Sciences de la Vie-Life Sciences 319 (10) 871-877

par Administrateur - publié le

Abstract :

In ras-transfected NIH3T3 cells, the transcription of the Mr 34,000 ß-galactoside specific lectin galectin-3 depends on transformation phenotypes. This observation suggests that this lectin is associated with the transformation process and/or that the ras oncogene may modulate its expression ; nevertheless the involvement of ras-gene product in galectin-3 gene expression still remains unclear. In the present study, we investigated the galectin-3 expression in human HOS cells transiently or stably transfected with a ras-containing vector We observed an increase in galectin-3 mRNA and protein content in stably ras-transfected cells which had lost their anchorage dependence for growth but no increase in cells which needed anchorage for growth or in transiently ras-transfected cells These results suggest that the galectin-3 up-regulation in ras-transfected HOS cells is the consequence of the cell transformation rather than a divert effect of the ras gene product on galectin-3 gene expression.