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Guerin, M ; Leng, M ; Rahmouni, AR

High resolution mapping of E-coli transcription elongation complex in situ reveals protein interactions with the non-transcribed strand

Embo Journal 15 (19) 5397-5407

par Administrateur - publié le , mis à jour le

Abstract :

We have used chemical probes and UV light to perform a high resolution mapping of an Escherichia coli transcription elongation complex that was arrested in vivo by a protein readblock at a position distal to the promoter. The in situ probing data provide a precise picture of a constrained ternary complex in which the front edge of the polymerase is located at