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Asseline, U ; Roig, V ; Thuong, NT

Modified oligonucleotides with a 5 ’-5 ’ interbase semi-rigid junction for alternate strand triplex formation

Tetrahedron Letters 39 (49) 8991-8994

par Administrateur - publié le

Abstract :

Solid-phase synthesis of 5’-5’-linked oligonucleotides with opposite polarities tethered via nucleic bases has been performed using a modified dinucleoside bearing an H-phosphonate group at the 3’-position of one nucleoside and a dimethoxytrityl group-at the 3’-position of the second nucleoside. This system is aimed at forming a base tetrad at the junction in order to provide better stabilization. The linker used between the two 5’-terminal bases in the same plane involves a triple bond in order to rigidify the junction. The two oligonucleotide :chains are either made of natural nucleosides or one of them is built With N3’ —>P5’ phosphoramidates. (C) 1998 Published by Elsevier Science Ltd. All rights reserved.