Partenaires

CNRS


Rechercher


Accueil > Publications > Recherche par années > Années 1990 > 1998

Abergel, C ; Rigal, A ; Chenivesse, S ; Lazdunski, C ; Claverie, JM ; Bouveret, E ; Benedetti, H

Crystallization and preliminary crystallographic study of a component of the Escherichia coli Tol system : TolB

Acta Crystallographica Section D-Biological Crystallography 54 102-104 Part 1

par Administrateur - publié le

Abstract :

TolB from Escherichia coli is part of the Tol system used by the group A colicins to penetrate and kill cells. A TolB derivative tagged with six histidines was overexpressed, purified by chelation on a nickel affinity column and crystallized using the SAmBA software to define the optimal crystallization protocol. The crystals belong to the monoclinic system, space group P2(1) with unit-cell parameters a = 64.48, b = 41.06, c = 78.41 Angstrom, beta = 110.78 degrees. Frozen crystals diffract to 1.9 Angstrom resolution. Screening for heavy-atom derivatives both on the native TolB and various cysteine-substituted mutants is in progress. In addition, a selenomethionine-substituted protein is being produced in order to use the MAD method for structure determination.