Accueil > Publications > Recherche par années > Années 1990 > 1998

Fournier, R ; Brule, F ; Segault, V ; Mougin, A ; Branlant, C

U3 snoRNA genes with and without intron in the Kluyveromyces genus : Yeasts can accommodate great variations of the U3 snoRNA 3 ’-terminal domain

Rna-A Publication of The Rna Society 4 (3) 285-302

par Administrateur - publié le

Abstract :

The U3 snoRNA coding sequences from the genomic DNAs of Kluyveromyces delphensis and four variants of the Kluyveromyces marxianus species were cloned by PCR amplification. Nucleotide sequence analysis of the amplification products revealed a unique U3 snoRNA gene sequence in all the strains studied, except for K. marxianus var. fragilis. The K. marxianus U3 genes were intronless, whereas an intron similar to those of the Saccharomyces cerevisiae U3 genes was found in K. delphensis. Hence, U3 genes with and without intron are found in yeasts of the Saccharomycetoideae subfamily. The secondary structure of the K. delphensis pre-U3 snoRNA and of the K. marxianus mature snoRNAs were studied experimentally.