Accueil > Publications > Recherche par années > Années 1990 > 1998

Toth, E ; Connac, F ; Helm, L ; Adzamli, K ; Merbach, AE

Direct assessment of water exchange on a Gd(III) chelate bound to a protein

Journal of Biological Inorganic Chemistry 3 (6) 606-613

par Administrateur - publié le

Abstract :

The Gd(III) complex of 4-pentylbicyclo[2.2.2]octane-1-carboxyl-di-L-aspartyl-lysine-derived DTPA, [GdL(H2O)](2-), binds to serum albumin in vivo, through hydrophobic interaction. A variable temperature O-17 NMR ? EPR, and Nuclear Magnetic Relaxation Dispersion (NMRD) study resulted in a water exchange rate of k(ex)(298) = 4.2 x 10(6) s(-1), and let us conclude that the GdL complex is identical to [Gd(DTPA)(H2O)](2-) in respect to water exchange and electronic relaxation. The effect of albumin binding on the water exchange rate has been directly evaluated by O-17 NMR.