Accueil > Publications > Recherche par années > Années 1990 > 1998

Midoux, P ; Kichler, A ; Boutin, V ; Maurizot, JC ; Monsigny, M

Membrane permeabilization and efficient gene transfer by a peptide containing several histidines

Bioconjugate Chemistry 9 (2) 260-267

par Administrateur - publié le

Abstract :

We designed a peptide, H5WYG (GLFHAIAHFIHGGWHGLIHGWYG), that permeabilizes cell membrane at a slightly acidic pH but not at neutral pH. Absorbance, fluorescence, and circular dichroism spectra showed that H5WYG undergoes a dramatic conformational change between pH 7.0 and 6.0 that correlates with the protonation of the histidyl residues. Cell permeabilization studies monitored by flow cytometry on living cells showed that H5WYG permeabilizes the cell membrane with a great efficiency at pH 6.4 but was not active at neutral pH ; at pH 6.8, the peptide permeabilized 50% of the cells at 20 degrees C within 10 min. H5WYG increased the expression of genes transferred to cells as glycosylated polylysine-DNA complexes, and the transfection efficiency was not impaired in the presence of serum.