Accueil > Publications > Recherche par années > Années 1990 > 1998

Kang, HC ; Ardourel, MY ; Guerin, B ; Monsigny, M ; Delmotte, FM

Purification of two lectins from a nopalin Agrobacterium tumefaciens strain

Biochimie 80 (1) 87-94

par Administrateur - publié le

Abstract :

Lectins were evidenced on the surface of one Agrobacterium tumefaciens wild strain (82.139) by agglutination test and neoglycoprotein labelling. Bacteria were incubated in the presence of various fluorescein-labelled neoglycoproteins and the binding was assessed by a fluorimetric method. Among the fluorescein-labelled neoglycoproteins tested, the one bearing a-D-galactosyl residues was the most efficient. The labelling was optimal at pH 5.0 and naught at pH above 7, The binding was specifically inhibited by homologous fluorescein-free neoglycoproteins. A galactoside-specific lectin was purified to homogeneity by affinity chromatography on agarose-A4 substituted with a-D-galactopyranosyl residues.