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Remy, E ; de Rocquigny, H ; Petitjean, P ; Muriaux, D ; Theilleux, V ; Paoletti, J ; Roques, BP

The annealing of tRNA(3)(Lys) to human immunodeficiency virus type 1 primer binding site is critically dependent on the NCp7 zinc fingers structure

Journal of Biological Chemistry 273 (9) 4819-4822

par Administrateur - publié le

Abstract :

The nucleocapsid protein NCp7 of the human immunodeficiency virus type 1 contains two zinc fingers of the CX2CX4HX4C type, flanked by several basic residues, and plays a major role in viral infectivity. Thus, NCp7 was shown to promote annealing of the tRNA(3)(Lys) to the primer binding site, a key step in reverse transcription. However ; previous in vitro experiments were unable to clarify the role of the zinc fingers in this process, due to nucleic acid aggregation induced by the basic N- and C-terminal domains of NCp7.