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Accueil > Publications > Recherche par années > Années 1990 > 1998

Roussel, A ; Yang, YQ ; Ferrato, F ; Verger, R ; Cambillau, C ; Lowe, M

Structure and activity of rat pancreatic lipase-related protein 2

Journal of Biological Chemistry 273 (48) 32121-32128

par Administrateur - publié le

Abstract :

The pancreas expresses several members of the lipase gene family including pancreatic triglyceride lipase (PTL) and two homologous proteins, pancreatic lipase-related proteins 1 and 2 (PLRP1 and PLRP2), Despite their similar amino acid sequences, PTL, PLRP1, and PLRP2 differ in important kinetic properties. PLRP1 has no known activity. PTL and PLRP2 differ in substrate specificity, bile acid inhibition, colipase requirement, and interfacial activation. To begin understanding the structural explanations for these functional differences, we solved the crystal structure of rat (r)PLRP2 and further characterized its kinetic properties.