Accueil > Publications > Recherche par années > Années 1990 > 1999

Maget-Dana, R ; Bureaud, N ; Schoentgen, F

Behaviour of the bovine brain phosphatidylethanolamine binding protein at the air/water interface

Materials Science & Engineering C-Biomimetic and Supramolecular Systems 8-943-46

par Administrateur - publié le

Abstract :

The cytosolic bovine brain PEBP is a basic protein of 21 kDa devoid of disulfide bridges. It has been shown to form stable monomolecular protein films at the air/water interface. In the course of time, the PEBP films expanded nearly three times probably as the result of protein unfolding at the interface. The PEBP diffusion from a protein solution towards the interface is very low in account of its high content in polar residues and of its widely ß-sheet structure. The adsorption rate of PEBP at the air/water interface is dependent on the bulk concentration of the protein and the saturation of the surface occurs as early as the bulk concentration reaches 2.5 x 10(-6) M in amino acid. The surface concentration of PEBP at saturation is less than 2 x 10(18) amino acid/m(2) a value in a 10 lower range than observed for Ether proteins of the same size and devoid of disulfide bridges. (C) 1999 Published by Elsevier Science S.A. All rights reserved.