Accueil > Publications > Recherche par années > Années 1990 > 1999

Carriere, V ; Piller, V ; Legrand, A ; Monsigny, M ; Roche, AC

The sugar binding activity of MR60, a mannose-specific shuttling lectin, requires a dimeric state

Glycobiology 9 (10) 995-1002

par Administrateur - publié le

Abstract :

MR60 is an intracellular membrane protein which has been shown to act as a mannoside specific lectin and to be identical to ERGIC-53, a protein characteristic of the endoplasmic reticulum-Golgi apparatus-intermediate compartment, acting as a shuttle. According to its primary sequence, this MR60/ERGIC-53 protein contains a luminal domain including the carbohydrate recognition domain, a stem, a transmembrane segment and a cytosolic domain. The endogenous MR60/ERGIC-53 protein is spontaneously oligomeric, (dimers and hexamers), In this paper, we study the relationship between the oligomerization state and the sugar binding capacity by using recombinant proteins.