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Coste, F ; Malinge, JM ; Serre, L ; Shepard, W ; Roth, M ; Leng, M ; Zelwer, C

Crystal structure of a double-stranded DNA containing a cisplatin interstrand cross-link at 1.63 angstrom resolution : hydration at the platinated site

Nucleic Acids Research 27 (8) 1837-1846

par Administrateur - publié le

Abstract :

cis-diamminedichloroplatinum (II) (cisplatin) is a powerful anti-tumor drug whose target is cellular DNA, In the reaction between DNA and cisplatin, covalent intrastrand and interstrand cross-links (ICL) are formed. Two solution structures of the ICL have been published recently. In both models the double-helix is bent and unwound but with significantly different angle values. We solved the crystal structure at 100K of a double-stranded DNA decamer containing a single cisplatin ICL, using the anomalous scattering (MAD) of platinum as a unique source of phase information.