Accueil > Publications > Recherche par années > Années 1990 > 1999

Carriere, V ; Landemarre, L ; Altemayer, V ; Motta, G ; Monsigny, M ; Roche, AC

Intradermal DNA immunization : Antisera specific for the membrane lectin MR60/ERGIC-53

Bioscience Reports 19 (6) 559-569

par Administrateur - publié le

Abstract :

The trafficking of intracellular membrane proteins in Golgi apparatus, endoplasmic reticulum or intermediate compartment has not yet been fully elucidated. The human MR60/ ERGIC-53 and the rat p58 proteins are one such protein ; and to study them in cell-free and in situ systems, high quality monospecific antisera are required. Highly specific antisera have been obtained after immunization of mice with plasmids containing a gene encoding either the full length or a truncated protein. The best results were obtained after intradermal injections of a plasmid encoding a truncated protein comprising both the luminal carbohydrate recognition domain and the stem down to a cysteine residue close to the C-terminal end, but neither the transmembrane nor the cytosolic domains. Such antisera have a very high titer and are very efficient tools to visualize the MR60 protein in situ or to selectively precipitate the MR60 proteins from a whole cell lysate.