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Bure, C ; Lelièvre, D ; Delmas, A

Identification of by-products from an orthogonal peptide ligation by oxime bonds using mass spectrometry and tandem mass spectrometry

Rapid Communications in Mass Spectrometry 14 (23) 2158-2164

par Administrateur - publié le

Abstract :

Synthetic proteins with unusual architecture are obtained through chemoselective ligation, a method based on the condensation of unprotected peptides under mild aqueous conditions,. The last step of a new procedure leading to a tri-branched conjugate consists of the chemoselective ligation reaction between an (amioooxy)acetyl peptide and a peptide aldehyde resulting from a first ligation via an oxime bond. In order to optimize the reaction conditions, electrospray ionization mass spectrometry combined with Liquid chromatography and tandem mass spectrometry has been used.