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Begusova, M ; Sy, D ; Charlier, M ; Spotheim-Maurizot, M

Radiolysis of nucleosome core DNA : a modelling approach

International Journal of Radiation Biology 76 (8) 1063-1073

par Administrateur - publié le

Abstract :

Purpose : To calculate the expected pattern of frank strand breaks (FSB) induced in the DNA of a nucleosome core particle (NCP) by the attack of radiolytic OH. radicals and to compare this with the experimental pattern of FSB induced by the in uitro irradiation of chicken NCP. Materials and methods : The structure of NCP was obtained from the PDB databank. Using molecular modelling, the structure of a linear DNA fragment with the central 60 bp sequence of NCP-DNA was determined. The accessibility of the sugar attack sites to OH. radicals at each nucleotide of the linear fragment or of the NCP-DNA was calculated. The probability of reaction of OH. with each sugar reactive sire was calculated using a Monte-Carlo method-based stochastic model.