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Serre, L ; de Jesus, KP ; Zelwer, C ; Bureaud, N ; Schoentgen, F ; Benedetti, H

Crystal structures of YBHB and YBCL from Escherichia coli, two bacterial homologues to a Raf kinase inhibitor protein

Journal of Molecular Biology 310 (3) 617-634

par Administrateur - publié le

Abstract :

In rat and human cells, RKIP (previously known as PEEP) was characterized as an inhibitor of the MEK phosphorylation by Raf-l. In Escherichia coli, the genes ybhb and ybcl possibly encode two RKIP homologues while in the genomes of other bacteria and archaebacteria other homologous genes of RKIP have been found. The parallel between the cellular signaling mechanisms in eukaryotes and prokaryotes suggests that these bacterial proteins could be involved in the regulation of protein phosphorylation by kinases as well. We first showed that the proteins YBHB and YBCL were present in the cytoplasm and periplasm of E. coli, respectively, after which we determined their crystallographic structures.