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Eon, S ; Culard, F ; Sy, D ; Charlier, M ; Spotheim-Maurizot, M

Radiation disrupts protein-DNA complexes through damage to the protein. The lac repressor-operator system

Radiation Research 156 (1) 110-117

par Administrateur - publié le

Abstract :

Binding of a protein to its cognate DNA sequence is a key step in the regulation of gene expression. If radiation damage interferes with protein-DNA recognition, the entice regulation profess may be perturbed. We have studied the effect of gamma rays on a model regulatory system, the E. coli lactose repressor-operator complex. We have observed the disruption of the complex upon irradiation in aerated solution. The complex is completely restored by the addition of nonirradiated repressor, hut not by the addition of nonirradiated DNA.