Accueil > Publications > Recherche par années > Années 2000 > 2001

Grivet, JP

NMR and Microorganisms

Current Issues in Molecular Biology 3 7-14.

par Administrateur - publié le

Abstract :

This article is an introduction to the use of NMR for the investigation of microbial physiology and metabolism. NMR parameters which determine the sensitivity and resolving power of the method are reviewed. A broad survey of current applications follows. Qualitative uses are described first ; they include compound identification and localisation. Quantitative aspects, such as pH, concentration and flux measurements are then examined, as well as the corresponding experimental constraints. The review ends with suggestions of possible future developments in instrument capabilities aimed at improving sensitivity : higher fields, spectroscopic and imaging microprobes.