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Cahours, X ; Tran, TT ; Mesplet, N ; Kieda, C ; Morin, P ; Agrofoglio, LA

Analysis of intracellular didanosine triphosphate at sub-PPB level using LC-MS/MS

Journal of Pharmaceutical and Biomedical Analysis 26 (5-6) 819-827

par Administrateur - publié le

Abstract :

An analytical procedure has been developed for the analysis of intracellular didanosine triphosphate (ddATP). An electrospray ionization tandem mass spectrometer (ESI-MS) was interfaced to liquid chromatography (LC) using a mobile phase CH3OH/H2O (25/75) containing 1% formic acid for the analysis of the 5’-triphosphate metabolite of the antiviral didanosine. In this procedure. ddATP was extracted from CEM-T4 cells, isolated using an exchange anion solid phase extraction procedure, enzymatically dephosphorylated and then analyzed by LC-MS/MS within a I min run time.