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Ohan, J ; Gilbert, MA ; Leseche, G ; Panis, Y ; Midoux, P ; Drouet, L

Nonviral gene transfer into primary cultures of human and porcine mesothelial cells

In Vitro Cellular & Developmental Biology-Animal 37 (7) 402-407

par Administrateur - publié le

Abstract :

Due to their abundance and accessibility, mesothelial cells may be suitable toots for recombinant reagent expression by gene transfer. Genetically modified porcine mesothelial cells (PMCs) may have the potential for the treatment of vascular diseases in humans. We studied the effect of various transfection reagents on the primary culture of PMCs and human mesothelial cells (HMCs). The cells were transfected with a plasmid encoding a reporter gene (luciferase or green fluorescent protein [GFP]) under the control of the cytomegalovirus promoter.