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Brule, F ; Marquet, R ; Rong, L ; Wainberg, MA ; Roques, BP ; Le Grice, SFJ ; Ehresmann, B ; Ehresmann, C

Structural and functional properties of the HIV-1 RNA-tRN(3)(Lys) primer complex annealed by the nucleocapsid protein : Comparison with the heat-annealed complex

Rna-A Publication of The Rna Society 8 (1) 8-15

par Administrateur - publié le

Abstract :

The conversion of the single-stranded RNA genome into double-stranded DNA by virus-coded reverse transcriptase (RT) is an essential step of the retrovirus life cycle. In human immunodeficiency virus type 1 (HIV-1), RT uses the cellular tRNA(3)(Lys) to initiate the (-) strand DNA synthesis. Placement of the primer tRNA(3)(Lys) involves binding of its 3’-terminal 18 nt to a complementary region of genomic RNA termed PBS. However, the PBS sequence is not the unique determinant of primer usage and additional contacts are important. This placement is believed to be achieved in vivo by the nucleocapsid domain of Gag or by the mature protein NCp. Up to now, structural information essentially arose from heat-annealed primer-template complexes (Isel et al., J Mol Biol, 1995, 247:236-250 ; Isel et al., EMBO J, 1999, 18:1038-1048).