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Charlier, M ; Eon, S ; Seche, E ; Bouffard, S ; Culard, F ; Spotheim-Maurizot, M

Radiolysis of lac repressor by gamma-rays and heavy ions : A two-hit model for protein inactivation

Biophysical Journal 82 (5) 2373-2382

par Administrateur - publié le

Abstract :

Upon gamma-ray or argon ion irradiation of the lac repressor protein, its peptide chain is cleaved and the protein loses its lac operator-binding activity, as shown respectively by polyacrylamide gel electrophoresis and retardation gel ejectrophoresis. We developed phenomenological models that satisfactorily account for the experimental results : the peptide chain cleavage model considers that the average number of chain breaks per protomer is proportional to the irradiation dose and that the distribution of the number of breaks per protomer obeys Poisson’s law.